This investigation was done to know the effect of Ginseng extract against Mycobacterium tuberculosis. They involved the growth of Mycobacterium tuberculosis in Dubos complete and basal broth containing 100,ug per ml of Ginseng extract, the Minimum Inhibitory Concentration of streptomycin, ethambutol and isoniazid in Dubes complete broth conta￢ining 100pg per ml, the effect of the experirnemtal tuberculosis on mice. The experimental groups were designed as followings.
The A group of mice was inoculated with M. tuberculosis in normal mice.
The B group of mice was simultaneously inoculated with M. tuberculosis and 20pg Ginseng extract per gm of body weight of mouse.
The C group of mice was given Ginseng extract for 15 days before inoculation with M. tuberculosis. After that these mice were inculated with only M. tuberculosis.
The D group of mice was given Ginseng extract throughout the experimental days and the mice were inoculated with M. tuberculosis at 15th experimental day.
The results were obtained as followings;
1. On the logarithmic growth phase, the promotion of the growth of M. tuberculosis; was not noticed in Dubos complete broth containing 100pg per ml of Ginseng extract. On the dead phase, _it was somewhat delayed in comparison with control. While the bacterial growth was inhibited in Dubos basal broth containing 100,ug per ml of Ginseng extract.
2. The minimum inhibitory concentration of lpg streptomycin did not differ from that of the control in Dubos complete broth containing 100pg per ml of Ginseng extract. The minimum inhibitory concentration of 2.5 pg ethambutol was lower a half fold than. that of control. The bacterial growth inhibited in the Ginseng extract containing 0.031/1g: isoniazid in compared to that of control.
3. Distributions of tubercle bacilli in each organ showed most highly in lungs and spleen, and did not do in liver, heart and kidneys. In group A tubercle bacilli were found more 20th experimental day than 10th day in experimental organs except heart.
But in other groups any similarities to the above were not found.
4. The spleen indices of M. tuberculosis in each experimental mouse did not signi￢ficantly differ from that of control.
From these above results, Ginseng extract did not promote the growth of M. tuber￢culosis but had an inclination to inhibit the growth of bacilli a little. The minimum inhibitory concentration might be lowered according to the antituberculosis agents in vitro. It might be commented that Ginseng extract did not influence in experimental tuberculosis, respectively.
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